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   1999| April  | Volume 42 | Issue 2  
    Online since October 12, 2009

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Acridine orange stained blood wet mounts for fluorescent detection of malaria.
N Hemvani, D S Chitnis, D S Dixit, M V Asolkar
April 1999, 42(2):125-8
The age old Romanowsky stained thick blood smear examination for malarial parasites may fail to reveal the low parasitaemia. The commercial 'QBC' like acridine orange stained capillary tube preparation has a limitation of precise species identification and the detection of extra-erythrocytic parasites. Hence, the present study was aimed to improve malarial parasite detection by using acridine orange to stain large blood drops in the form of wet coverglass mounts. The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent microscope and the results compared with the Leishman's stained thick blood smears in a blind study. The positivity of malarial parasites reported by the modified acridine orange staining was 248 against 109 by Leishman's stained thick blood smears. The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, allows scanning of larger blood volume (75 ul) at lower magnification and the morphological details at higher magnification helps to make the precise species identification.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  935 251 -
Quality assurance programme in haematology at a teaching hospital in the eastern region of Nepal.
S P Sah, G A Raj, M B Prakash
April 1999, 42(2):145-9
Quality assurance in haematology laboratory is intended to ensure the reliability of the laboratory tests. A quality assurance programme has two main aspects, namely, internal quality control and external quality assessment. A two year experience of quality assurance in haematology laboratory at B.P. Koirala Institute of Health Sciences, Nepal, is presented here. As a part of internal quality control, test results in the laboratory were scrutinized before release on a daily basis. Inconsistent result were checked for the given values with control material. In addition, the laboratory is a participant of the 'External Haematology Quality Assurance Programme' conducted by WHO regional reference centre at AIIMS, New Delhi, India. Variations related to errors in manual and autopipetting, calibration and inter-observer differences have been noted from time to time and rectified. The programme has helped us to deliver quality service in haematology laboratory at BPKIHS.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  908 148 -
Evaluation of thyroid hormones in chronic renal failure.
A Xess, A Gupta, U Kumar, H P Sharma, K M Prasad
April 1999, 42(2):129-33
When chronic renal failure becomes advanced, the serum levels of most hormones are altered because of several interplaying mechanisms. This study was carried out to evaluate the level of total thyroxine (T4), triiodothyronine (T3) and thyrotropin (TSH) in 96 clinically euthyriod patient with chronic renal failure and 25 healthy individual as control. The patients were grouped into two groups, 62 patient on conservative management and 34 patients on chronic haemodialysis. The patient of both groups showed significant decrease in total T3 and T4 level as compared to normal control. Serum TSH level were similar in both groups as well as in control and were with in normal limit except 3 patients on conservative management which showed TSH level above normal chronic haemodialysis did not have positive effect in alteration of serum T3, T4 and TSH level.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  739 121 -
Diagnostic distinction between unusual malignant tumors of gall bladder by histochemistry and antigenic phenotype.
S Agarwal, S Gupta, M Sikka
April 1999, 42(2):151-4
In a review of 79 cases of gall bladder malignancy, nineteen cases were labelled as unusual tumors while sixty were diagnosed as adenocarcinoma. Alcian blue, PAS, Grimelius' and Masson trichrome stains were done. Expression of EMA, CEA and desmin was assessed (PAP). Histological subtype was revised, in eleven cases out of 19. Five tumors initially diagnosed as squamous cell carcinoma were found to be positive for mucin and CEA and hence were reclassified as adenosquamous carcinoma. Three undifferentiated carcinomas and two malignant carcinoids were labelled as adenocarcinoma and composite tumor respectively. Positive reactivity with CEA and alcian blue PAS and absence of AFP helped in differentiating one giant cell carcinoma from hepatocellular carcinoma. No definite marker could be identified in one case of malignant mesenchymal tumor. Histochemistry and immunohistochemistry also helped in confirming the diagnosis of three cases of carcinoma in situ, one of malignant carcinoid and three of clear cell carcinoma.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  707 87 -
Cloning and expression of a cell wall associated protein gene of Mycobacterium tuberculosis.
A J Mohamed, G Jayaraman
April 1999, 42(2):135-43
Genomic library of Mycobacterium tuberculosis (SIHV) was constructed into T7 promoter based vector pBluescript II KS (+/-) in Escherichia coil. The expression of mycobacterial antigens from recombinants was detected by immunoscreening using E-coli-preabsorbed hyperimmune rabbit anti-M tuberculosis cell wall associated proteins serum. Among 980 clones tested by immunoscreening, a clone pJJ5 was found to produce mycobacterial antigen. This clone was further characterized by immunoblotting and found to produce a 36 kDa of cell wall associated antigen Detection of antibodies response to this antigen in the serum of the majority of the individuals with tuberculosis indicates that this antigen is capable of stimulating a humoral immune response.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  704 86 -
Medullary thyroid carcinoma with melanin production--a case report.
Z N Singh, R Ray, N Kumar, M Aron, S D Gupta
April 1999, 42(2):159-63
Melanin production in medullary thyroid carcinomas is rare. The present case illustrates melanin and other atypical features of medullary carcinoma of the thyroid in a fifty year old female. The diagnosis was suggested on the cytomorphological features seen on fine needle aspiration cytology smears. On histo-pathological examination the tumor was extensively pigmented with frequent mitosis. Amyloid was conspicuously scarce. Confirmation of diagnosis was done by immunohistochemical positivity for calcitonin and HMB-45 on tissue sections. The case is being presented in view of its rarity and distinct immunoreactivity. Review of literature is done and the implications of such dual positivity in the histogenesis and divergent phenotype of this tumor are discussed.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  727 56 -
Diagnosis of hydatid disease of abdomen and thorax by ultrasound guided fine needle aspiration cytology.
A Singh, Y Singh, V K Sharma, A K Agarwal, D Bist
April 1999, 42(2):155-6
Five cases of hydatid disease of abdomen and thorax were diagnosed by fine needle aspiration cytology (FNAC) under ultrasound guidance. The age of patients ranged from 25-50 years, with a median age of 37 years. Male to female ratio was 2:3. None of the cases were clinically diagnosed as hydatid disease, but ultrasonography raised suspicion in two cases. The location of cyst in four cases was liver and one aspiration was done from lung. Smears from all cases showed features consistent with hydatid disease. None of the patients showed any untoward allergic reaction following FNAC.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  672 86 -
Neonatal candidial endocarditis--a rare manifestation of systemic candidiasis.
P Vaideeswar, A Sivaraman, J R Deshpande
April 1999, 42(2):165-8
Infective candidial endocarditis in four premature neonates is reported. These occurred as a complication of systemic candidiasis. Vegetations were present on the right side of the heart in two cases and both sides in the other two. Diagnosis requires a high degree of clinical suspicion. Mortality rate is high despite appropriate therapy.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  615 50 -
Retroperitonial, teratoma as fetus in fetu--a case report.
S K Shrivastava, H P Sharma, S K Sinha, K M Prasad
April 1999, 42(2):169-70
We report a rare case of Retroperitonial teratoma containing Axial Skeleton long bone. Jaw, pelvis & scapula in a 27 yrs old male. Aberration in monozygotic twinning may rarely present as Fetus in Fetu. Rarer is presentation in Adult.
[ABSTRACT]   Full text not available  [PDF]  [PubMed]
  574 57 -
An overview of PSA/free PSA with special reference to recent trends in diagnosis of prostatic cancer.
S R Goyal, V H Talib, S K Khurana
April 1999, 42(2):171-8
Full text not available  [PDF]  [PubMed]
  425 70 -
Phase contrast microscopy : utility in obstetrics and gynaecology.
S K Shahi, S Ranga, S K Khurana, V H Talib
April 1999, 42(2):123-4
Full text not available  [PDF]  [PubMed]
  380 42 -
Prevalence of Acinetobacter anitratus in urinary isolates at G.M and associated hospitals.
G Banerjee, S K Agarwal
April 1999, 42(2):181-181
Full text not available  [PDF]  [PubMed]
  331 34 -
Redefining status of pathology and microbiology.
A Upreti
April 1999, 42(2):179-179
Full text not available  [PDF]  [PubMed]
  333 29 -
Pneumatosis cystoides intestinalis--a case report.
S Mutha, D T Kumbhalkar, S K Bobhate
April 1999, 42(2):157-8
Full text not available  [PDF]  [PubMed]
  324 37 -
Redefining status of pathology and microbiology.
M J Dave
April 1999, 42(2):180-180
Full text not available  [PDF]  [PubMed]
  288 32 -
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