Indian Journal of Pathology and Microbiology

ORIGINAL ARTICLE
Year
: 2012  |  Volume : 55  |  Issue : 2  |  Page : 175--179

HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization


Hemlata S Shirsat, Sridhar Epari, Tanuja Shet, Rajani Bagal, Rohini Hawaldar, Sangeeta B Desai 
 Division of Molecular Pathology, Department of Pathology, Tata Memorial Hospital and Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Mumbai, India

Correspondence Address:
Sridhar Epari
Department of Pathology, Tata Memorial Hospital and Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Mumbai, Maharashtra
India

Introduction : HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH). Recent studies have shown chromogenic in-situ hybridization (CISH) as a relatively cheaper alternative. Materials and Methods : Forty-three nonconsecutive, randomly selected primary invasive breast cancer cases were evaluated for c-erbB-2 (HER2 protein) by IHC and gene amplification by FISH and CISH. Results of each of the same were compared. Results : CISH showed approximately 90% and 100% concordance for IHC negative and positive cases, respectively; while approximately 94.4% and 91% concordance with FISH amplified and non-amplified cases, respectively. Conclusion : This study showed feasibility of incorporation of CISH as a low cost option in routine management of breast carcinoma in the Indian setting. Secondly, reconfirmation of IHC negative and positive cases can be done by CISH.


How to cite this article:
Shirsat HS, Epari S, Shet T, Bagal R, Hawaldar R, Desai SB. HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization.Indian J Pathol Microbiol 2012;55:175-179


How to cite this URL:
Shirsat HS, Epari S, Shet T, Bagal R, Hawaldar R, Desai SB. HER 2 status in invasive breast cancer: Immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization. Indian J Pathol Microbiol [serial online] 2012 [cited 2022 Aug 20 ];55:175-179
Available from: https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=2;spage=175;epage=179;aulast=Shirsat;type=0