Indian Journal of Pathology and Microbiology


Year
: 2001  |  Volume : 44  |  Issue : 4  |  Page : 399--401

Evaluation of semi-quantitative methods for protein and sugar estimation in urine.


S Chaturvedi, N Jain, A Bhatia 
 University College of Medical Sciences, Delhi

Correspondence Address:
S Chaturvedi
University College of Medical Sciences, Delhi

OBJECTIVES: To compare the accuracy of semi quantitative methods for estimation of protein and sugar in urine as shown by their agreement with the quantitative estimation. Hundred randomly collected samples of urine were analysed for levels of protein and sugar. Protein estimation was dine by dipstick and sulphosalicylic acid method (SSA) and sugar by dipstick and Benedict«SQ»s semi-quantitative methods. Kappa analysis was done on Epi Info 6.03 software to assess the agreement of these semi quantitative methods with the quantitative estimation. Neither of the two tests for urine protein, dipstick or SSA, showed good agreement with the quantitative estimation (Kappa coefficient: 0.26 and 0.07 respectively). However, the dipstick was significantly better than SSA (p < 0.05). For urine sugar, both dipstick and Benedict«SQ»s tests showed good agreement with the quantitative estimation (Kappa coefficient: 0.78 and 0.84 respectively). The difference between them was insignificant. Results demonstrate that for urine protein, dipstick or SSA show poor agreement with quantitative values. For urine sugar estimation, Benedict«SQ»s semi-quantitative test shows good agreement with the quantitative values and is as good as the dipstick method.


How to cite this article:
Chaturvedi S, Jain N, Bhatia A. Evaluation of semi-quantitative methods for protein and sugar estimation in urine. Indian J Pathol Microbiol 2001;44:399-401


How to cite this URL:
Chaturvedi S, Jain N, Bhatia A. Evaluation of semi-quantitative methods for protein and sugar estimation in urine. Indian J Pathol Microbiol [serial online] 2001 [cited 2022 Aug 11 ];44:399-401
Available from: https://www.ijpmonline.org/article.asp?issn=0377-4929;year=2001;volume=44;issue=4;spage=399;epage=401;aulast=Chaturvedi;type=0