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Expression of protein phosphatase 4 in different tissues under hypoxia
Yanyan Ma1, Jing Hou2, Dengliang Huang2, Yaogang Zhang2, Zhe Liu2, Meiyuan Tian1
1 Central Laboratory, Affiliated Hospital of Qinghai University, Tongren Road 29; Medical College of Qinghai University, Xining, Qinghai Province, China
2 Central Laboratory, Affiliated Hospital of Qinghai University, Tongren Road 29, China
Correspondence Address:
Meiyuan Tian,
The Central Laboratory of Qinghai University Affiliated Hospital; Qinghai University, Nanchuan Xi Road 158th, Chengzhong, Xining City, Qinghai Province - 810000
China
 Source of Support: None, Conflict of Interest: None DOI: 10.4103/ijpm.ijpm_1179_21
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Relevant research data shows that there is a certain degree of energy metabolism imbalance in highland residents. Protein phosphatase 4 (PP4) has been found as a new factor in the regulation of sugar and lipid metabolism. Here, we investigate the differential expression of PP4 at a simulated altitude of 4,500 m in the heart, lung, and brain tissues of rats. A hypoxic plateau rat model was established using an animal decompression chamber. A blood routine test was performed by an animal blood cell analyzer on rats cultured for different hypoxia periods at 4,500 m above sea level. Quantitative polymerase chain reaction (qPCR) and western blot were used to detect the changes of protein phosphatase 4 catalytic subunit (PP4C) gene and protein in heart, lung, and brain tissues. The PP4C gene with the highest expression level found in rats slowly entering the high altitude area (20 m-2200 m-7 d-4500 m-3 d) was about twice as high as the low elevation group (20 m above sea level). The simulated high-altitude hypoxia induced an increase of PP4C expression level in all tissues, and the expression in the lung tissue was twice as expressed as heart and brain tissue at high altitude (P < 0.05). These results suggest that the PP4 phosphatase complex is ubiquitously expressed in rat tissues and likely involved in adaptation to or disease associated with high-altitude hypoxia.
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