| Abstract|| |
Ganglioglioma is a slow-growing relatively low-grade mixed glioneuronal tumor with most cases corresponding to the WHO Grade I category. It frequently presents with seizures. The temporal lobe is the most common location followed by frontal, parietal, and occipital lobes. These generally behave in a benign fashion and have a favorable prognosis. We describe a case of a 24-year-old male presenting with convulsions and a calcified parieto-occipital mass. This mass removed from the parietal lobe showed neoplastic glial and dysplastic neuronal tissue amidst extensive areas of calcification and foci of ossification. On immunohistochemistry, the glial component expressed glial fibrillary acidic protein whereas the dysplastic neuronal component expressed synaptophysin and CD34. Epithelial membrane antigen was negative and Ki-67 showed a low proliferative index. After the surgery, the patient is free of neurological symptoms. Widespread calcification and ossification are very unusual in ganglioglioma, which prompted us to report this case.
Keywords: Calcification, ganglioglioma, ossification
|How to cite this article:|
Kavishwar VS, Chadha KG, Barodawala SM, Murthy AK. Unusual ganglioglioma with extensive calcification and ossification. Indian J Pathol Microbiol 2016;59:395-7
|How to cite this URL:|
Kavishwar VS, Chadha KG, Barodawala SM, Murthy AK. Unusual ganglioglioma with extensive calcification and ossification. Indian J Pathol Microbiol [serial online] 2016 [cited 2022 Jan 22];59:395-7. Available from: https://www.ijpmonline.org/text.asp?2016/59/3/395/188115
| Introduction|| |
Ganglioglioma corresponds to the WHO Grade I and the WHO classifies it in mixed glioneuronal tumors. It is a well-differentiated, slow-growing glioneuronal tumor composed of neoplastic mature ganglion cells and neoplastic glial cells. It may occur in any age group, temporal lobe being the most common location. In this patient, the calcified mass removed from the parieto-occipital lobe showed neoplastic glial and dysplastic neuronal tissue amidst extensive areas of calcification and foci of ossification. This type of widespread calcification and ossification is very unusual in ganglioglioma, which prompted us to report this case.
| Case Report|| |
A 24-year-old Indian male working in the Middle East complained of repeated attacks of abnormal movements of the right side of the body for 1 week with an episode of loss of consciousness. He had a fall during these convulsions and injured his face and skull. There was no previous history of trauma. Speech and ophthalmologic examination was within normal limits. The clinical impression was tuberculoma.
Computed tomography and magnetic resonance imaging showed a calcified left parieto-occipital mass measuring 16 mm × 16 mm, which was hypointense on T1- and T2-weighted images and nonenhancing on postcontrast with mild perifocal vasogenic edema and erosion of the left inner table. There were no other lesions in the brain.
The differential diagnoses were (1) encephalomalacia/dystrophic calcification (2) low-grade nonenhancing neoplastic space occupying lesion. A spectroscopic examination of the aforementioned area was advised.
The surgeon noted a very unusual appearance of the lesion having three distinct components - a solid chunk of bony hard mass, an amorphous component, and typical soft glial element [Figure 1]a.
|Figure 1: (a) Solid calcified chunk of tissue within the brain parenchyma (intraoperative). (b) Neuronal disorganization, binucleation, and prominent nucleoli (H and E, ×400). (c) Ossification with trabeculae formation and calcification (H and E, ×100). (d) Extensive calcification (H and E, ×100)|
Click here to view
The tissue was received as excised in three parts: tissue from thickened arachnoid, a calcified subcortical mass, and amorphous subcortical tissue.
Sections showed lamellated calcospherites [Figure 1]b amidst cortical tissue. There was neuronal disorganization with occasional cytomegaly [Figure 1]c. Extensive calcification was also noted along the vessel wall. A distinct glial component was identified. Eosinophilic granular bodies were also seen [Figure 2]a. Admixed bony trabeculae were identified [Figure 1]d. No necrosis, abnormal mitoses, or anaplastic features were noted in any of the sections. Widespread calcification and formation of bony trabeculae are very rare findings.
|Figure 2: (a) H and E, showing eosinophilic granular bodies. (b) Ganglion cells showing positivity for synaptophysin (×400). (c) Ganglion cells showing positivity for CD34 (×400). (d) glial fibrillary acidic protein positivity (×100)|
Click here to view
Glial fibrillary acidic protein (GFAP), synaptophysin, CD34, epithelial membrane antigen (EMA), and Ki-67 (Labvision) immunohistochemical stains were performed using the UltraVision Quanto HRP detection system on the Thermo Scientific Lab Vision 480S Autostainer.
The glial component expressed GFAP [Figure 2]d whereas the dysplastic neuronal component expressed synaptophysin and CD34 [Figure 2]b and c. EMA was negative. Ki-67 showed a low proliferation index.
Hence, a diagnosis of ganglioglioma of WHO Grade I was made.
After the surgery, the patient is free of neurological symptoms.
| Discussion|| |
Gangliogliomas are uncommon neoplastic lesions which represent 1.3–2% of all brain tumors.,, Nair et al. reported an incidence of 0.32% in their 5-year study of 1560 cases. The age of patients is variable ranging from 2 months to 70 years. Data from a large series with a total of 626 patients indicate an average age at diagnosis ranging from 8.5 to 25 years, with only a slight male preponderance. Our patient was a 24-year-old young male.
Majority of cases occur in the temporal lobe (>70%) and present with seizures., These also occur in the frontal, parietal, and occipital lobes.,, In our case, the tumor was located in the parieto-occipital lobe and the patient presented with convulsions. Nair et al. also reported a case in the parietal lobe in their series of five cases.
Intracranial calcification is a common finding in neuro-radiology, associated with a broad spectrum of disorders., However, the morphology of a mixture of neuronal and glial cell elements as seen in our case is characteristic of ganglioglioma. In addition, abundant lamellated calcospherites and metaplastic ossification were also observed, which is unusual and rare. Makariou and Patsalides and Kiroglu et al. report that 35–50% of all gangliogliomas show calcification., In the study by Nair et al., all cases showed calcification and perivascular lymphocytes. Like us, Hori et al. also reported a case of ganglioglioma containing osseous tissue.
On immunohistochemistry (IHC), neuronal proteins such as microtubule-associated protein 2, NeuN, neurofilaments, and synaptophysin are useful to demonstrate the neuronal component, and GFAP highlights the glial component. CD34 positivity is also observed in the neuronal component. Our case showed a similar immunophenotype.
Heavily calcified localized tumefactive lesions in the brain have relatively limited differential diagnoses. Among the non-neoplastic lesions, meningiomatosis and vascular malformation calcifying pseudotumor of neuraxis can be considered, but display characteristic pathological features, which were not present in our case.
Among the neoplastic lesions, psammomatous and rarely metaplastic meningioma are important differentials. The concentric lamellated calcification may coalesce and dense calcification can almost completely replace meningothelial cells. Here, concentric lamellated calcification was seen, but there were no meningothelial cells, and tumor cells were negative for EMA.
Other primary intra-axial tumors known for extensive calcification include oligodendroglioma and less frequently subependymal giant cell tumor, ependymoma, choroid plexus papilloma, craniopharyngioma, neurocytoma, supratentorial primitive neuroectodermal tumor, and metastatic tumors. However, histomorphology and clinicoradiological findings were not supportive of any of these.
Thus, based on the gross, histomorphology, and IHC, the diagnosis of ganglioglioma of parieto-occipital region of WHO Grade I was established. Because of its uncommon location, typical morphology but with abnormal ossification and calcification, and rarity of reports in world literature, we are eager to share this unusual case.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Becker AJ, Wiestler OD, Figarella-Branger, D Blumcke I. Neuronal and mixed neuronal – Glial tumours. In: Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, editors. World Health Organization Classification of Tumours of the Nervous System. 4th
ed., Ch. 6. Lyon, France: International Agency for Research on Cancer (IARC) Press; 2007. p. 103-6.
Nair V, Suri VS, Tatke M, Saran RK, Malhotra V, Singh D. Gangliogliomas: A report of five cases. Indian J Cancer 2004;41:41-6.
Makariou E, Patsalides A. Intracranial calcifications. Appl Radiol 2009;38(11):48-60.
Kiroglu Y, Calli C, Karabulut N, Oncel C. Intracranial calcifications on CT. Diagn Interv Radiol 2010;16:263-9.
Hori A, Weiss R, Schaake T. Ganglioglioma containing osseous tissue and neurofibrillary tangles. Arch Pathol Lab Med 1988;112:653-5.
Aiken AH, Akgun H, Tihan T, Barbaro N, Glastonbury C. Calcifying pseudoneoplasms of the neuraxis: CT, MR imaging, and histologic features. AJNR Am J Neuroradiol 2009;30:1256-60.
Huang J, Petersson F. Intracerebral metaplastic meningioma with prominent ossification and extensive calcification. Rare Tumors 2011;3:e20.
Dr. Vikas Shashikant Kavishwar
Department of Histopathology, Metropolis Healthcare Limited, Central Laboratory, 4th Floor, Commercial Building - 1A, Kohinoor Mall, Vidyavihar (West), Mumbai - 400 070, Maharashtra
Source of Support: None, Conflict of Interest: None
[Figure 1], [Figure 2]