Indian Journal of Pathology and Microbiology
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Year : 2013  |  Volume : 56  |  Issue : 1  |  Page : 20-23

Anti-Toxoplasma gondii antibody detection in serum and urine samples by enzyme-linked immunosorbent assay in HIV-infected patients

1 Department of Microbiology, KGMU, Lucknow, Uttar Pradesh, India
2 Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Sayan Bhattacharyya
481, P. Majumdar Road, Kolkata -700 078, West Bengal
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0377-4929.116143

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Background: Toxoplasmosis is a common parasitic infection of man, and reactivation of latent disease in HIV-infected patients can cause fatal encephalitis. Diagnosis depends on demonstration of parasite-specific antibodies in serum. In HIV-infected patients, IgM is often undetectable, whereas IgG remains detectable in the majority. Urine sample is very easily available and has not been evaluated for immunodiagnosis of toxoplasmosis. Aim: The study was an effort to find whether urine sample can be used in place of serum for immunodiagnosis of toxoplasmosis. Materials and Methods: Enzyme-linked immunosorbent assay (ELISA) was carried out in serum and urine samples collected from 100 HIV-infected patients to detect anti-toxoplasma IgG and IgM antibodies and whether positivity correlated with the CD4 T-cell counts of patients. Results: In this study, we observed that there was no significant difference in positivity of anti-toxoplasma IgM and IgG between serum and urine samples of HIV-infected patients by ELISA. There was a negative correlation between CD4 count and seropositivity. Conclusion: Urine sample can be satisfactorily used in place of serum for immunodiagnosis of toxoplasmosis.

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