Indian Journal of Pathology and Microbiology
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Year : 2012  |  Volume : 55  |  Issue : 1  |  Page : 47-51

Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method

Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang, Malaysia

Correspondence Address:
Zainoodin Sheik Abdul Kader
Advanced Medical and Dental Institute, Universiti Sains Malaysia, Lot 8, Persiaran Seksyen 4/1, Bandar Putra Bertam, 13200 Kepala Batas, Penang
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Source of Support: Advanced Medical and Dental Institute Research Incentive, Conflict of Interest: None

DOI: 10.4103/0377-4929.94855

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Background: Platelets release more than 30 cytokines to provide primary hemostatic function. In addition, platelets are also known to release antimicrobial peptides upon activation by thrombin. Materials and Methods: In this study, comparative analysis of antibacterial activity of activated and non-activated expired platelet concentrate was determined against Gram-positive and Gram-negative bacteria by Kirby-Bauer disk diffusion method. Thrombin was used to prepare activated platelet concentrate. Gram-positive bacteria tested in this study were S.aureus and S.pyogenes and Gram-negative bacteria were E.coli and K.oxytoca. All the bacteria used in this study were sensitive strains from clinical isolates. Activated and non-activated platelet showed no zone of inhibition against S.pyogenes and E.coli. Results: Activated platelet showed antibacterial activity against S.aureus and K.oxytoca with the zone of inhibition of 8.3 ± 0.6 mm and 7.7 ± 0.2 mm, respectively. Zone of inhibition observed in non-activated platelet against S.aureus and K.oxytoca were 7.8 ± 0.4 mm and 7.5 ± 0.3 mm, respectively. Conclusions: These findings showed that no significant differences in antibacterial activity produced by activated and non-activated platelet. However, zone of inhibition observed in activated and non-activated platelet indicate the presence of antibacterial property in expired platelet.

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