| ORIGINAL ARTICLE |
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| Year : 2009 | Volume
: 52
| Issue : 1 | Page : 59-61 |
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Transport and storage of sputum specimen by using cetylpyridinium chloride for isolation of mycobacteria
Nita Pal, Babita Sharma, Bharti Malhotra, Suman Rishi
Department of Microbiology, SMS Medical College, Jaipur, Rajasthan, India
Correspondence Address:
Nita Pal
82, Green Nagar, Durgapura, Jaipur
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0377-4929.44966
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Of the 191 sputum specimens that were collected from pulmonary tuberculosis patients, 78.65% (140/178) specimens were culture positive when processed within 48 h by the NaOH method. The culture positivity in the same specimen that were stored with cetylpyridinium chloride (CPC) and processed after 7-8 days was 70.22% (125/178), whereas those stored without CPC and processed by the NaOH method was 46.62% (83/178). The difference in number of positive cultures obtained before storage and after storage (without CPC) was statistically significant (P = 0.001). Culture positivity by the CPC method was comparable with that of NaOH method before storage and the difference was not statistically significant (P = 0.35). |
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